Ruminant Nutrition Lab

Manual of Laboratory Techniques

Sample Grinding Procedure PDF (20 kb)

Dry Matter and Ash CURRENT PDF (27 kb)

60°C Dry Matter Instructions CURRENT PDF (10 kb)

pH and VFA Analysis PDF (10 kb)

Particle Size Analysis PDF (45 kb)

Glassware Cleaning Procedure PDF (20 kb)

Cleaning Gooch Crucibles PDF (19 kb)

Nitrogen

Nitrogen Analysis CURRENT PDF (19 kb)

Ammonia Nitrogen PDF (20 kb)

Neutral/Acid Detergent Insoluble Nitrogen PDF (15 kb)

Pepsin Insoluble Nitrogen PDF (14 kb)

AOAC Pepsin Insoluble Nitrogen PDF (11 kb)

Ficin Activity Determination PDF (24 kb)

Protease – Pepsin Insoluble Nitrogen PDF (31 kb)

Pepsin – Pancreatin Insoluble Nitrogen PDF (30 kb)

Soluble Nitrogen – Wise Burroughs Method PDF (15 kb)

Soluble Nitrogen – Sodium Chloride Method PDF (11 kb)

Non-Protein Nitrogen PDF (18 kb)

Escape Protein from Omasal Samples PDF (21 kb)

Fiber

Neutral Detergent Fiber CURRENT PDF (16 kb)

Acid Detergent Fiber CURRENT PDF (15 kb)

ADF/NDF Ankom Technique CURRENT PDF (23 kb)

Sulfuric Acid Lignin CURRENT PDF (15 kb)

Permanganate Lignin PDF (16 kb)

Suggested Fiber Publications PDF (16 kb)

In Vitro Ruminal Fermentation

In Vitro Dry Matter Disappearance CURRENT PDF (27 kb)

In Vitro Starch Disappearance PDF (33 kb)

In Vitro NDF Digestion PDF (23 kb)

In Vitro Ammonia Nitrogen Release PDF (20 kb)

In Vitro Gas Production PDF (20 kb)

In Situ

Standard In Situ Procedure for Concentrates CURRENT PDF (27 kb)

Standard In Situ Procedure for Forages CURRENT PDF (29 kb)

In Situ NDIN PDF (19 kb)

In-Situ Pepsin-HCl Digestion CURRENT PDF (15 kb)

Others

Megazyme Starch Analysis-simplified CURRENT PDF (46 kb)

• Megazyme Starch Analysis  PDF (168 kb)

Phosphorous Analysis of Feeds  PDF (26 kb)

Sodium Analysis – Electrode PDF (19 kb)

HPLC Lactate and VFA  PDF (16 kb)

Fat and NDF Extraction CURRENT  PDF (23 kb)

H2S Collection Procedures PDF (23 kb)

Toluene Procedure  PDF (79 kb)

Toluene Water Analysis CURRENT PDF (17 kb)

%Sulfur Determination CURRENT  PDF (24 kb)

Markers

Titanium Dioxide CURRENT  PDF (29 kb)

DAPA Determination CURRENT  PDF (58 kb)

Cobalt – EDTA  PDF (29 kb)

Polyethylene Glycol  PDF (31 kb)

Indigestible Acid Detergent Fiber  PDF (16 kb)

Acid Insoluble Ash PDF (14 kb)

Binding Rare Earths PDF (22 kb)

Rare Earth Element Analysis  PDF (16 kb)

Marker Calculations  PDF (13 kb)

Blood Flow Calculations  PDF (10 kb)

Modified Purine Assay  PDF (33 kb)

Diaminopimelic Acid  PDF (18 kb)

Xylose Determination  PDF (15 kb)

Metabolites

Ruminal Fluid VFA PDF (25 kb)

Analysis of Rumen Ammonia PDF (26 kb)

Creatinine PDF (19 kb)

Blood

Plasma Urea Nitrogen PDF (20 kb)

Deproteinization of Plasma PDF (10 kb)

Blood Urea Nitrogen PDF (32 kb)

Microorganisms

Counting Ruminal Protozoa PDF (33 kb)

Bacteria Counting PDF (18 kb)

Tables

Buffer pKa and pH Range Values PDF (27 kb)

Mead Feed Analysis

Laboratory Safety

Laboratory Rules and Information

Standard Procedures and Safety Rules  PDF (20 kb)

Laboratory Safety  PDF (24 kb)

Disposal of Biohazards  PDF (10 kb)

Incompatible Chemicals  PDF (31 kb)

Chemical Waste Pick-up  PDF (20 kb)

General Rules for use of Pipette  PDF (13 kb)

Basic Rules for Handling an Enzyme  PDF (15 kb)

Centrifuge Safety PDF (25 kb)

Autoclave Safety PDF (24 kb)

Heating Device Safety PDF (18 kb)

Housekeeping in Labs PDF (13 kb)

Data Collecting, Reporting, and Computing PDF (14 kb)

Notebook Checklist PDF (11 kb)

Periodic Table of the Elements PDF (44 kb)

Laboratory Safety Signiture Sheet
Laboratory Safety Presentation

Online Training
EHS Virtual Manual Assesment

• Click on: Access Your Profile
• Enter email address: jmacdonald2@unl.edu

EHS Safety Training

• Go through each training module assigned. Print off assessment after passing quiz and turn into Ruminant Nutrition Lab.

IACUC Training