Bikash Ranjan Sahoo

Education
2008 - 2013: Integrated MSc. in Biotechnology, KIIT School of Biotechnology, KIIT
University, Bhubaneswar, Odisha, India.
2015 - 2021: Graduate Research Assistant, School of Veterinary Medicine and Biomedical
Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
2021- contd: Post-Doctoral Research Associate, Department of Animal Science, University
of Nebraska-Lincoln.

Technical Skills

• Experienced in handling small laboratory animals (blood collection, tissue and organ harvest, intraperitoneal and sub-cutaneous injections, etc.)
• Cell culture: Experienced in handling various mammalian and insect cell lines.
• Virus culture and infection (Zika virus, Vaccinia virus, Vesicular Stomatitis virus)
• Molecular Cloning, siRNA knockdown
• Protein Expression and Purification, SDS-PAGE and Western Blotting
• Flow cytometry and Confocal Microscopy
• Seahorse metabolomics flux analysis

Awards, Posters and Presentations

• Graduate Research Assistantship, University of Nebraska-Lincoln, 2015-Present
• Presented poster titled ‘Zika virus induces oxidative stress in neural progenitor cells’ in FLYSWAT, 2018
• Oral Presentation titled ‘Mammalian Target of Rapamycin (mTOR) signaling is required for Zika virus (ZIKV) replication’ in FLYSWAT, 2019
• Received the Milton E Mohr award for the year 2020-2021.

Publications

• Bikash R. Sahoo, Aryamav Pattnaik, Crook A, Powers R, Rodrigo Franco, Asit K. Pattnaik: Redox Regulation and Metabolic Dependency of Zika Virus Replication (in preparation for PLOS Pathogens)
• Prativa Majee, Aryamav Pattnaik, Bikash R. Sahoo, Uma Shankar, Asit K. Pattnaik, Amit Kumar, Debasis Nayak. Inhibition of Zika Virus Replication by G-Quadruplex Binding Ligands. Molecular Therapy – Nucleic Acids, 2021 Mar 5;23 (691-701).
• Bikash R. Sahoo, Aryamav Pattnaik, Arun S. Annamalai, Rodrigo Franco, Asit K. Pattnaik: Mechanistic Target of Rapamycin (mTOR) Signaling Activation Antagonizes Autophagy to Facilitate Zika Virus Replication. Journal of Virology, 2020 Oct 27;94(22). Cover Page Illustration Journal of Virology, 2020 Oct 27;94(22).
• Aryamav Pattnaik*, Bikash R. Sahoo*, Asit K. Pattnaik: Current Status of Zika Virus Vaccines: Successes and Challenges, Vaccines (Basel), 2020 May 31;8(2):E266. * Equal contribution.
• Arun S. Annamalai, Aryamav Pattnaik, Bikash R. Sahoo, Zack P. Guinn, Brianna L. Bullard, Eric A. Weaver, David Steffen, Sathish Kumar Natarajan, Thomas M. Petro and Asit K. Pattnaik: An Attenuated Zika Virus Encoding Non-Glycosylated Envelope (E) and Non-Structural Protein 1 (NS1) Confers Complete Protection against lethal Challenge in a Mouse Model, Vaccines, 2019 Sep;12;7(3).
• Pattnaik A, Palermo N, Sahoo BR, Yuan Z, Hu D, Annamalai AS, Vu HLX, Correas I, Prathipati PK, Destache CJ, Li Q, Osorio FA, Pattnaik AK, Xiang SH: Discovery of a nonnucleoside RNA polymerase inhibitor for blocking Zika virus replication through in silico screening, Antiviral Research, 2018 Mar;151:78-86.
• Annamalai AS, Pattnaik A, Sahoo BR, Muthukrishnan E, Natarajan SK, Steffen D, Vu HLX, Delhon G, Osorio FA, Petro TM, Xiang SH, Pattnaik AK: Zika Virus Encoding Nonglycosylated Envelope Protein Is Attenuated and Defective in Neuroinvasion, Journal of Virology, 2017 Nov 14;91(23). Selected as ‘Spotlight Article’ for Journal of Virology, 2017 Nov 14;91(23).